Sir Bradley Wiggins says he "100%" did not cheat and claims he is the victim of an attempt to "smear" him.
A report by MPs said Wiggins and Team Sky "crossed an ethical line" by using drugs that are allowed under anti-doping rules to enhance performance instead of just for medical purposes.
"Not at any time in my career did we cross the ethical line," Wiggins told BBC sports editor Dan Roan.
"I refute that 100%. This is malicious, this is someone trying to smear me."
Five-time Olympic champion Wiggins, the first Briton to win the Tour de France, said he is the subject of a "witch hunt", that his children "get a hammering at school" which is "disgusting to witness", and that it is "a living hell".
He added: "[Cycling] is the most scrutinised sport in the world. I can't control what people are going to think but for some people, whatever you do it is not going to be enough. I just don't know any more in this sport - you are damned if you do, damned if you don't.
"The widespread effect it has had on the family is just horrific. I am having to pick up the pieces with the kids - I would not wish it on anyone."
Wiggins, 37, was granted therapeutic use exemption (TUEs) to take the corticosteroid triamcinolone, which can treat allergies and respiratory issues, shortly before the 2011 Tour de France, his 2012 Tour win and the 2013 Giro d'Italia.
Wiggins says he used the drug on just one other occasion, denying the suggestion in the report that he had used it up to nine times across a four year period.
The Digital, Culture, Media and Sport Committee (DCMS) report said it received "confidential material from a well-placed and respected source" about Team Sky's medical policy between 2011 and 2013 that states Wiggins and a smaller group of riders trained separately from the rest of the team in preparation for the 2012 season.